Mrs. Pfahnl’s Bio A Lab: Catalase
September 29, 2020
Purpose: Investigate catalase activities in different fruits or vegetables.
Materials:
1. Hydrogen peroxide 3% from any drug store2. Dish washing detergent any brand
3. Potato (white, yellow or sweet) or carrot
4. Avocado
5. Apple
6. Tap water
7. Three narrow transparent cups labeled 1-3
8. measuring cup
9. measuring spoon: 1 table spoon
10. ruler
11. cutting board
12. knife
Procedures:
1. Add half cup of water to each cup
2. Add one drop of dish washing detergent into each cup and mix
3. Add 1 table spoon hydrogen peroxide 3% into each cup and mix
4. cut potato/carrot, avocado and apple into 1 cm cubes
5. Add 6 cubes of potato/carrot into “cup 1”
6. Add 6 cubes of avocado into “cup 2”
7. Add 6 cubes of apple into “cup 3”
8. Measure the thickness of the foam at 3 min, 20 min
Thickness of foam at 3 min, cup 1=0.5 cm, cup 2=1 cm, cup 3 a few bubbles.
Thickness of foam at 3 min, cup 1=1 cm, cup 2=2 cm, cup 3 not much bubbles.
Observation and data analysis
1. Take pictures to document your experiment (34% of your grade)
2. Make data chart and graph for each cup. X= time Y=thickness of foam (33% of your grade)
3. Make Flipgrid video to explain the observation. Which plant material has the most catalase activity and which one has the least? (33% of your grade)